Comparative Genomic Analysis of Biofilm-Forming Polar Microbacterium sp. Strains PAMC22086 and PAMC21962 Isolated from Extreme Habitats

The members of Microbacterium isolated from different environments are known to form peptidoglycan. In this study, we compared the biofilm-forming abilities sp. PAMC22086 (PAMC22086), which was soil in South Shetland Islands and PAMC21962 (PAMC21962), algae Islands. analysis average nucleotide identity phylogeny revealed a 97% similarity oxydans VIU2A, while showed 99.1% hominis SGAir0570. For comparative genomic PAMC21962, genes related biofilm formation were identified using EggNOG KEGG pathway databases. possessed by both cpdA, phnB, rhlC, glgC, regulate virulence, formation, multicellular structure. Among indirectly involved unlike csrA, glgB, responsible for attachment glycogen biosynthesis. Additionally, PAMC22086, additional functional rsmA, is mobility polysaccharide production, dksA, GTPase, oxyR, play roles cell cycle stress response, identified. addition, ability two isolates examined vivo standard crystal violet staining technique, morphological differences investigated. It evident distribution biofilm-associated between strains that bacteria can survive niches employing distinct strategies. Both exhibit morphologies. forms attaches side, indicates growth starting center. formation-related not well understood. However, it has been observed species regardless number they possess. Through comparison species, specific core adhesion, plays crucial role formation. This study provides comprehensive profile genus’s features preliminary understanding genus, laying foundation further research.